Publications

Analysis of the Pythium ultimum transcriptome using Sanger and Pyrosequencing approaches

Cheung F, Win J, Lang JM, Hamilton J, Vuong H, Leach JE, Kamoun S, André Lévesque C, Tisserat N, Buell CR

PMID: 19014603

Abstract

Pythium species are an agriculturally important genus of plant pathogens, yet are not understood well at the molecular, genetic, or genomic level. They are closely related to other oomycete plant pathogens such as Phytophthora species and are ubiquitous in their geographic distribution and host rage. To gain a better understanding of its gene complement, we generated Expressed Sequence Tags (ESTs) from the transcriptome of Pythium ultimum DAOM BR144 (= ATCC 200006 = CBS 805.95) using two high throughput sequencing methods, Sanger-based chain termination sequencing and pyrosequencing-based sequencing-by-synthesis.