Publications

BMC genomics. 2010-06-11; 11.374.

Reassociation kinetics-based approach for partial genome sequencing of the cattle tick, Rhipicephalus (Boophilus) microplus

Guerrero FD, Moolhuijzen P, Peterson DG, Bidwell S, Caler E, Bellgard M, Nene VM, Djikeng A

PMID: 20540747

Abstract

The size and repetitive nature of the Rhipicephalus microplus genome makes obtaining a full genome sequence fiscally and technically problematic. To selectively obtain gene-enriched regions of this tick's genome, Cot filtration was performed, and Cot-filtered DNA was sequenced via 454 FLX pyrosequencing.

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