Publications

Cytogenetic and genome research. 2007-01-01; 119.3-4: 268-74.

Isolation and characterization of the highly repeated fraction of the banana genome

Hribová E, Dolezelová M, Town CD, Macas J, Dolezel J

PMID: 18253041

Abstract

Although the nuclear genome of banana (Musa spp.) is relatively small (1C approximately 610 Mbp for M. acuminata), the results obtained from other sequenced genomes suggest that more than half of the banana genome may be composed of repetitive and non-coding DNA sequences. Knowledge of repetitive DNA can facilitate mapping of important traits, phylogenetic studies, BAC-based physical mapping, and genome sequencing/annotation. However, only a few repetitive DNA sequences have been characterized in banana. In this work, we used DNA reassociation kinetics to isolate the highly repeated fraction of the banana genome (M. acuminata 'Calcutta 4'). Two libraries, one prepared from Cot

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