Publications

Microbiology resource announcements. 2025-05-30; e0029825.

Marine microbial mock communities for validating rRNA gene amplicon sequencing

Lampe RH, Rabines AJ, Ellman BA, Zheng H, Allen AE

PMID: 40444952

Abstract

Mock communities are defined mixtures of cells or DNA for methods development, testing, or validation. Here, we present mock communities based on previously used 16S and 18S rRNA gene sequences from diverse marine microorganisms. Their use as controls for amplicon sequencing enables the detection of biased or aberrant runs.

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