Publications

Multiple displacement amplification as an adjunct to PCR-based detection of Staphylococcus aureus in synovial fluid

Kathju S, Lasken RS, Satish L, Johnson S, Stoodley P, Post JC, Ehrlich GD

PMID: 20942932

Abstract

Detection of bacterial nucleic acids in synovial fluid following total joint arthroplasty with suspected infection can be difficult; among other technical challenges, inhibitors in the specimens require extensive sample preparation and can diminish assay sensitivity even using polymerase chain reaction (PCR)-based methods. To address this problem a simple protocol for prior use of multiple displacement amplification (MDA) as an adjunct to PCR was established and tested on both purified S. aureus DNA as well as on clinical samples known to contain S. aureus nucleic acids.